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In vivo cyclo phosphamide mediated augmentation and aqueous human gamma globulin induced suppression of murine delayed hyper sensitivity to human gamma globulin are reflected by in vitro human gamma globulin stimulated proliferation of lymph node cells from equivalent mice



In vivo cyclo phosphamide mediated augmentation and aqueous human gamma globulin induced suppression of murine delayed hyper sensitivity to human gamma globulin are reflected by in vitro human gamma globulin stimulated proliferation of lymph node cells from equivalent mice



Cellular Immunology 52(2): 404-413



To determine if immunological processes which culminate in in vivo expression, augmentation and suppression of delayed effector cell activity are mirrored by events that can be quantified in vitro, the previously characterized murine model of delayed hypersensitivity (DHS), which employs SJL/J mice immunized with aggregated human .gamma.-globulin (AHGG) in complete Freund's adjuvant (CFA), was employed. Lymph node cells (LNC) from cyclophosphamide (CY)-pretreated, AHGG-CFA immunized mice expressed increased proliferation in the presence of HGG and concanavalin A (Con A) but decreased LPS [lipopolysaccharide] responsiveness compared with LNC from equivalently immunized but non-CY-treated animals. LNC from CY-treated, AHGG-CFA immunized mice that were pretreated with aqueous HGG (aqHGG), but not aqueous bovine serum albumin, evidenced a markedly decreased capacity to proliferate in the presence of HGG compared with LNC from equivalent animals that were not pretreated with aqHGG. This suppressive effect was not due to antibody production. In vitro quantitation of antigen-induced proliferation by LNC from HGG-DHS mice appears to correlate with modulatory effects which are observed in in vivo expression of DHS responses.

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