Increased phosphorylation of myosin light chain kinase after an increase in cyclic AMP in intact smooth muscle

de Lanerolle, P.; Nishikawa, M.; Yost, D.A.; Adelstein, R.S.

Science 223(4643): 1415-1417


ISSN/ISBN: 0036-8075
PMID: 6322302
DOI: 10.1126/science.6322302
Accession: 005664492

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The role of cAMP-mediated phosphorylation of myosin L chain kinase in relaxing smooth muscle was examined. The kinase was immunoprecipitated from tissue extracts and the phosphate content was determined. The addition of forskolin to resting or methacholine-contracted muscles resulted in an increase in myosin L chain kinase phosphorylation and a relaxation of contracted muscles. Evidently, phosphorylation of myosin L chain kinase is 1 of the reactions in the process by which cAMP causes relaxation of smooth muscle.