Section 6
Chapter 5,665

Increased radioresistance of cells in cultured multicell spheroids. II. Kinetic and cytogenetic studies

Hinz, G.; Dertinger, H.

Radiation and Environmental Biophysics 21(4): 255-264


ISSN/ISBN: 0301-634X
PMID: 6867266
DOI: 10.1007/bf01341462
Accession: 005664826

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Using the Chinese hamster cell line B14 FAF 28, several specific properties of the contact effect (CE) of radiation action in spheroids were investigated. CE protected the spheroid cells against several types of radiation-induced cytogenetic misfunctions such as blockage in S and G2 + M-phase, mutagenesis and chromosome damage. Repair of DNA strand-breaks was the same in monolayers and spheroids. CE is a property of the single cell and does not depend on the proliferative status (cycling or noncycling) of the cells. Apparently, CE is the result of a physiological modification of the cells occurring during growth in the 3-dimensional spheroid matrix and requiring metabolic cooperation and cAMP for its induction.

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