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Influence of cyto kinins and temperature on development of asparagus plumosus shoot tips in vitro



Influence of cyto kinins and temperature on development of asparagus plumosus shoot tips in vitro



Physiologia Plantarum 45(1): 73-76



Lateral shoot tips from young shoots of A. setaceus (Kunth) Jessop (syn. A. plumosus Baker) were grown on a modified Murashige and Skoog medium. PBA [6-(benzylamino)-9-(2-tetrahydropyranyl)-9H-purine] was the most effective cytokinin for growth and development of A. plumosus shoot tips. The optimal range of PBA was between 0.02 and 2 mg/l. Zeatin was less effective, and kinetin, BA [6-benzylaminopurine] and 2iP [6-(3-methyl-2-buten-1-ylamino)-purine] were poor as cytokinins for A. plumosus. The optimal temperature for growth and development was 21.degree. C. There was interaction between temperature and PBA concentration. With increasing temperature from 17-24.degree. C the need for PBA increased from 0.2-2 mg/l.

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