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Influence of organic phosphates on 3 phosphoglycerate dependent oxygen evolution in 3 carbon pathway and 4 carbon pathway mesophyll chloroplasts



Influence of organic phosphates on 3 phosphoglycerate dependent oxygen evolution in 3 carbon pathway and 4 carbon pathway mesophyll chloroplasts



Plant and Cell Physiology 28(5): 805-814



In C4 plants phosphoenolpyruvate (PEP) of the C4 cycle may be transported on a chloroplast transporter which also transports 3-phosphoglycerate (3-PGA) and triosephosphates. In C3 plants PEP is not considered to be effectively transported on the chloroplast phosphate translocator. The influences of certain organic phosphates, having a similar structure of either PEP or triose-phosphates, on 3-PGA dependent O2 evolution by C4 (Digitaria sanquinalis L. Scop.) and C3 (Hordeum vulgare L.) mesophyll chloroplasts were investigated. In the C4 mesophyll chloroplasts phosphoglycolate was a competitive inhibitor (Ki = 2.1 mM) of 3-PGA dependent O2 evolution, and was as effective as previously reported for PEP. 2-Phosphoglycerate was also a competitive inhibitor (Ki = 8.6 mM) of O2 evolution of the C4 mesohyll chloroplasts with 3-PGA as substrate, while phospholactate was a weak inhibitor and glyphosate had no effect. Neither PEP, phosphoglycolate nor 2-phosphoglycerate were effective inhibitors of 3-PGA dependent O2 evolution in the C3 chloroplasts. Phosphohydroxypyruvate was a competitive inhibitor of 3-PGA dependent O2 evolution in both chloroplasts types. The selectivity in inhibition of O2 evolution with 3-PGA as substrate suggests that the C4 mesophyll chloroplasts can recognize certain organic phosphates with the phosphate in the C-2 or C-3 position but that the C3 mesophyll chloroplasts can only effectively recognize certain organic phosphates with the phosphate in the C-3 position. The results also support the view that 3-PGA and PEP are transported on the same phosphate translocator in C4 mesophyll chloroplasts.

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