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Inheritance and heredity and characteristics of sources of resistance to helminthosporium maydis race t obtained through mutagenesis on texas male sterile cytoplasm maize



Inheritance and heredity and characteristics of sources of resistance to helminthosporium maydis race t obtained through mutagenesis on texas male sterile cytoplasm maize



Annales de l'Amelioration des Plantes (Paris) 27(6): 753-766



In order to obtain CmsT plants susceptible to H. maydis race T (HmT), 2 kinds of mutagenesis were carried out on seeds of the French line F7T: exposure to .gamma. rays (15,000 R) and soaking in a 2.5 g/l ethyl methane sulfonate solution (EMS). The plants from the treated seeds are called M1 generation; crossed with a restorer line (F71) it produces a male fertility progeny called M2 giving M3 by selfing. In order to screen for resistance to HmT, M3 seedlings were checked either with the cultured filtrate medium or by direct inoculation of conidia. Tests reveal resistant plants from 42 M2 progenies (6.7%) after EMS treatment and from only one M2 progeny after .gamma. ray irradiation. In all cases one M2 plant carrying resistance trait appears to segregate resistant and susceptible plants in its M3 progeny. This resistance trait appears to be transmitted in M4 progenies for only 15 families. In M5, plants are grown in fields with climatic conditions favorable to the fungus. After this drastic screening only 6 families with a level of resistance equal or superior to normal cytoplasm line survived. Two kinds of male sterility are obtained: the Texas type corresponding to the original type, characterized by pollen abortion and normally developed tassels; F7N maintains the male-sterile phenotype; a modified male-sterile type characterized by tassel abnormalities and non-viable pollen grams; F7N does not maintain the male-sterile phenotype. In whole resistant families the 2nd type is more frequent than the 1st one. The existence of male fertile plants bearing no restorer alleles suggests that reversion towards fertility is probably due to a cytoplasmic event. Moreover the reversed male fertile plants are always resistant to HmT. Adequate crosses emphasize the resistant trait of 6 families screened in M5 is not transmitted through pollen grain fertilization. Resistance is then supposed to be maternally inherited. The behavior of some plants after toxin inoculation, or that of their isolated mitochondria with toxin, suggests the existence of intermediary resistance levels. The comparison between results obtained after in vivo mutagenesis treatment and those of Gengenbach et al. after promoting plants from calli grown in the presence of toxin show that it is possible to change some characteristics of the Texas cytoplasm such as male-sterility and susceptibility to HmT; both traits are closely related and tend to be preserved simultaneously.

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Accession: 005697851

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Related references

Heredity and characteristics of sources of resistance to Helminthosporium maydis race T, obtained by mutagenesis in maize with Texas male sterile cytoplasm. Annales de l' Amelioration des Plantes 27(6): 753-766, 1977

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