+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Inhibition of oleandomycin synthesis by glucose



Inhibition of oleandomycin synthesis by glucose



Antibiotiki i Meditsinskaya Biotekhnologiya 30(8): 579-584



The results of the study on the effect of glucose and various carbohydrates on biosynthesis of oleandomycin by Streptomyces antibioticus are presented. It was found that glucose added at the beginning or by the 48th hour of the fermentation process on the complex medium inhibited oleandomycin biosynthesis. To investigate the mechanism of the glucose effect, a fermentation medium was developed. It provided variation of the carbohydrate composition, determination of the protein content in the culture and evaluation of the mycelium productivity. With the use of this medium it was shown that monosaccharides such as galactose, fructose and glucose significantly activated the mycelium growth as compared to lactose and sucrose. At the same time glucose completely inhibited oleandomycin biosynthesis when added either as an only carbohydrate component or in combination with galactose or fructose, while the presence of the other two monosaccharides did not prevent antibiotic production, though the mycelium productivity is lowered as compared to that with the use of the disaccharide. Therefore, the inhibitory effect of glucose on biosynthesis of oleandomycin was not connected with activation of the culture growth by it. Acidification of the medium on cultivation of the streptomycete in the presence of glucose only partially explained its inhibitory effect, since inhibition was maintained on the medium with addition of CaCO3, which stabilizes pH. Addition of 2-deoxy-D-glucose, a nonmetabolized glucose analog, to the fermentation medium retarded antibiotic production. It is possible that the inhibitory effect of glucose on biosynthesis of oleandomycin is not associated with its metabolism.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 005705696

Download citation: RISBibTeXText


Related references

Inhibition of ohmefentanyl on articular afferent-induced responses of neurons in the spinal lamina X. Zhongguo Yao Li Xue Bao 13(2): 150-152, 1992

Synthesis of 2 formyl adenosine using di ethoxy acetonitrile as a synthon. Heterocycles 16(8): 1315-1320, 1981

Synthesis of a biospecific adsorbent for the purification of the three human retinoic acid receptors by affinity chromatography. Biochemical and Biophysical Research Communications 187(2): 711-716, 1992

Synthesis of the infectious pancreatic necrosis virus polyprotein, detection of a virus-encoded protease, and fine structure mapping of genome segment A coding regions. Journal of Virology 61(12): 3655-3664, 1987

Inhibition of oleandomycin biosynthesis by glucose. Antibiotiki i Meditsinskaia Biotekhnologiia 30(8): 579-584, 1985

Oleandomycin and its natural and synthetic analogs i. dissociative ionization oleandomycin and its derivatives. Antibiotiki i Khimioterapiya 34(3): 194-199, 1989

Evidence for the coordinate control of glycogen synthesis, glucose utilization, and glycolysis in Escherichia coli. I. Quantitative covariance of the rate of glucose utilization and the cellular level of fructose 1,6-diphosphate during exponential growth and nutrient limitation. Journal of Biological Chemistry 250(18): 7188-7193, 1975

Evidence for the coordinate control of glycogen synthesis glucose utilization and glycolysis in escherichia coli part 1 quantitative covariance of the rate of glucose utilization and the cellular level of fructose 1 6 di phosphate during exponential growth and nutrient limitation. Journal of Biological Chemistry 250(18): 7188-7193, 1975

Glucose sensing issues for the artificial pancreas. Journal of Diabetes Science and Technology 2(4): 732-734, 2008

Acute inhalation toxicity of T-2 mycotoxin in the rat and guinea pig. Fundamental and applied toxicology: official journal of the Society of Toxicology 14(1): 54-59, 1990

Hepatic effects of breathing pure oxygen for eight months upon rats, dogs and monkeys. Aerosp Med 38(3): 273-274, 1967

Inhibition of protein synthesis by ethanol in isolated rat liver parenchymal cells. Biochimica et Biophysica Acta 474(2): 312-320, 1977

Inhibition by 2-Br alpha-ergocryptine-mesilate (CB-154) of suckling-induced pituitary prolactin depletion in lactating rats. Experientia 30(10): 1173-1173, 1974

Combined antibiotic therapy of tropical infections with PA 765 and PA 775. Antibiotics Annual 1956: 63-66, 1956

Production and investigation of a new antifungal antibiotic Abstract. ACTA PHYSIOL ACAD SCI HUNGARICAE 16(Suppl ): 79-80, 1959