Home
  >  
Section 6
  >  
Chapter 5,707

Inhibition of purified Escherichia coli leader peptidase by the leader (signal) peptide of bacteriophage M13 procoat

Wickner, W.; Moore, K.; Dibb, N.; Geissert, D.; Rice, M.

Journal of Bacteriology 169(8): 3821-3822

1987

ISSN/ISBN: 0021-9193
PMID: 3301818
DOI: 10.1128/jb.169.8.3821-3822.1987
Accession: 005706687
The leader peptide of bacteriophage M13 procoat inhibited the cleavage of M13 procoat or pre-maltose-binding protein by purified Escherichia coli leader peptidase. This finding confirms inferences that the leader is the primary site of enzyme recognition and suggests a rationale for the rapid hydrolysis of leader peptides in vivo.

PDF emailed within 0-6 h: $19.90




Related References

Date, T.; Wickner, W. 1981: Isolation of the Escherichia coli leader peptidase gene and effects of leader peptidase overproduction in vivo Proceedings of the National Academy of Sciences of the United States of America 78(10): 6106-6110
Wolfe, P.B.; Wickner, W.; Goodman, J.M. 1983: Sequence of the leader peptidase gene of Escherichia coli and the orientation of leader peptidase in the bacterial envelope Journal of Biological Chemistry 258(19): 12073-12080
Shen, L.M.; Lee, J.I.; Cheng, S.Y.; Jutte, H.; Kuhn, A.; Dalbey, R.E. 1991: Use of site-directed mutagenesis to define the limits of sequence variation tolerated for processing of the M13 procoat protein by the Escherichia coli leader peptidase Biochemistry 30(51): 11775-11781
Mandel, G.; Wickner, W. 1979: Translational and post-translational cleavage of M13 procoat protein: extracts of both the cytoplasmic and outer membranes of Escherichia coli contain leader peptidase activity Proceedings of the National Academy of Sciences of the United States of America 76(1): 236-240
Watts, C.; Silver, P.; Wickner, W. 1981: Membrane assembly from purified components. II. Assembly of M13 procoat into liposomes reconstituted with purified leader peptidase Cell 25(2): 347-353
Richards, J.H. 1985: Model substrate for leader peptidase a synthetic 73 amino acid residue peptide m 13 procoat Journal Of Cellular Biochemistry Supplementt B: 124
Zwizinski, C.; Wickner, W. 1980: Purification and characterization of leader (signal) peptidase from Escherichia coli Journal of Biological Chemistry 255(16): 7973-7977
Dalbey, R.E.; Wickner, W. 1988: Characterization of the internal signal-anchor domain of Escherichia coli leader peptidase Journal of Biological Chemistry 263(1): 404-408
Tokunaga, M.; Loranger, J.M.; Wolfe, P.B.; Wu, H.C. 1982: Prolipoprotein signal peptidase in Escherichia coli is distinct from the M13 procoat protein signal peptidase Journal of Biological Chemistry 257(17): 9922-9925
Dalbey, R.E.; Kuhn, A.; Wickner, W. 1987: The internal signal sequence of Escherichia coli leader peptidase is necessary, but not sufficient, for its rapid membrane assembly Journal of Biological Chemistry 262(27): 13241-13245
Zhu, H.; Dalbey, R.E. 1989: Both a short hydrophobic domain adn a carboxyl-terminal hydrophilic region are important for signal function in the Escherichia coli Leader peptidase The Journal of Biological Chemistry 264(20): 11833-11838
Zhu, H.Y.; Dalbey, R.E. 1989: Both a short hydrophobic domain and a carboxyl-terminal hydrophilic region are important for signal function in the Escherichia coli leader peptidase Journal of Biological Chemistry 264(20): 11833-11838
Kuo, D.W.; Chan, H.K.; Wilson, C.J.; Griffin, P.R.; Williams, H.; Knight, W.B. 1993: Escherichia coli leader peptidase: production of an active form lacking a requirement for detergent and development of peptide substrates Archives of Biochemistry and Biophysics 303(2): 274-280
Paul, L.K.; Izaguirre, G.; Hansen, J.N. 1999: Studies of the subtilin leader peptide as a translocation signal in Escherichia coli K12 FEMS Microbiology Letters 176(1): 45-50
Kuhn, A.; Wickner, W. 1985: Conserved residues of the leader peptide are essential for cleavage by leader peptidase Journal of Biological Chemistry 260(29): 15914-15918