Inhibition of sterol biosynthesis and stem elongation of tobacco nicotiana tabacum cultivar turkish samson seedlings induced by some hypo cholesterolemic agents

Douglas, T.J.; Paleg, L.G.

Journal of Experimental Botany 32(126): 59-68


ISSN/ISBN: 0022-0957
Accession: 005707508

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Incorporation of DL-[2-14C]mevalonic acid ([2-14C]MVA) into 4-desmethylsterols in N. tabacum cv. Turkish Samson seedlings was inhibited by tris-(2-diethylaminoethyl)phosphate hydrochloride (SK&F 7997-A3), tris(2-dimethylaminoethyl)phosphate hydrochloride (SK&F 7732-A3), trans-1,4-bis(2-chlorobenzylaminomethyl)cyclohexane dihydrochloride (AY 9944) and the plant growth retardant 2-isopropyl-4-dimethylamino-5-methylphenyl-1-piperidine carboxylate methyl choloride (Amo 1618). Reductions in 4-desmethylsterol levels resulted from treatment with AY 9944 and Amo 1618, but not the SK&F compounds. Amo 1618 and SK&F 7997-A3 both significantly reduced the specific activity of each of the 4 major 4-desmethylsterols examined. Although SK&F 7732-A3 reduced the specific activity of campesterol and AY 9944 reduced the specific activity of stigmasterol, neither had an effect on the specific activity of .beta.-sitosterol. Stem elongation of tobacco seedlings was retarded by SK&F 7997-A3, AY 9944 and SK&F 7732-A3, particularyl the former, and the retarded plants thus produced were morphologically indistinguishable from the Amo 1618-treated plants. Application of exogenous stigmasterol, or GA3, to the chemically-retarded plants resulted in a reversal of stem growth retardation.