+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Inhibitors in human urine of radio immunoassay for the detection of gonococcal antigens



Inhibitors in human urine of radio immunoassay for the detection of gonococcal antigens



Journal of Medical Microbiology 12(2): 177-186



Several substances in urine inhibited the radioimmunoassay of added gonococcal antigens [Ag]. The supernatants of 2/3 of urine samples from male patients with gonorrhea or non-specific urethritis (NSU) were inhibitory. The inhibitory caused by many samples was reduced or completely abolished by the addition of soybean trypsin inhibitor (STI); STI-sensitive inhibition is probably due to proteolytic enzymes, probably from pus cells. Their inhibitory effect was due to their action on gonoccocal Ag and not on antibodies in the assay system. Some supernatants contained other inhibitors unaffected by STI; some of these were dialyzable and others were not. Sediments from the urine of patients with NSU or gonorrhea were often strongly inhibitory but treatment with STI annulled all but very slight inhibition. STI-treated sediments could be used in an assay designed to detect gonococcal Ag.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 005709034

Download citation: RISBibTeXText


Related references

Detection of gonococcal antigens in human urine by radio immunoassay. Journal of Medical Microbiology 12(2): 161-176, 1979

Inhibitors in urine of radioimmunoassay for the detection of gonococcal antigens. Journal of Medical Microbiology 12(2): 177-185, 1979

Detection of human ia antigens by a cellular radio immunoassay. Canadian Federation of Biological Societies Proceedings 22: 73, 1979

Toxicological detection of pholcodine and its metabolites in urine and hair using radio immunoassay fluorescence polarization immunoassay enzyme immunoassay and gas chromatography mass spectrometry. International Journal of Legal Medicine 104(1): 43-46, 1990

Development of a rapid qualitative radio immunoassay for the detection of human chorionic gonadotropin in urine. Clinical Chemistry 28(7): 1613, 1982

Radio immunoassay and enzyme immunoassay for the detection of candida albicans antigens. Zentralblatt fuer Bakteriologie Mikrobiologie und Hygiene 1 Abt Originale A 251(4): 469, 1982

The asymptomatic female detection of antibody activity to gonococcal pili antigen by radio immunoassay. Abstracts of the Annual Meeting of the American Society for Microbiology 76: C149, 1976

Detection of gonococcal antigens in urine by radioimmunoassay. Journal of Medical Microbiology 12(2): 161-175, 1979

Time course of detection of oxymorphone oxycodone hydromorphone hydrocodone naloxone and naltrexone in human urine by radio immunoassay and emit opiate screening tests. Pharmacologist 22(3): 170, 1980

Diagnosis of HIV-1 infection by detection of antibody IgG to HIV-1 in urine with ultrasensitive enzyme immunoassay (immune complex transfer enzyme immunoassay) using recombinant proteins as antigens. Journal of Clinical Laboratory Analysis 8(4): 237-246, 1994

Detection of antibody IgG to HIV-1 in urine by sensitive enzyme immunoassay (immune complex transfer enzyme immunoassay) using recombinant proteins as antigens for diagnosis of HIV-1 infection. Journal of Clinical Laboratory Analysis 7(6): 353-364, 1993

Radio immunoassay for detection of immuno globulin g antibodies against enterobacterial antigens. Clinical Research 28(2): 383A, 1980

Detection of arenavirus antigens and antibodies by solid phase radio immunoassay. Voprosy Virusologii (4): 459-462, 1981

Enzyme immunoassay of C1q and its application to the detection of C1q antigens in urine. Journal of Clinical and Laboratory Immunology 16(3): 169-172, 1985

Protein a radio immunoassay for detection of circulating immune complexes and identification of antigens. Annals of the Rheumatic Diseases 41(2): 197, 1982