+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Inhibitory effects of interferon on mouse megakaryocytic progenitor cells in culture



Inhibitory effects of interferon on mouse megakaryocytic progenitor cells in culture



Experimental Hematology 8(8): 1048-1056



Mouse bone marrow cells were grown in plasma clots and megakaryocyte formation was stimulated with human urinary erythropoietin (Ep). The expression of megakaryocyte colony forming units (CFUM) and of single megakaryocyte forming units (M) was evaluated after 7 days in culture. Usually 10 times more M than CFUM were found. Megakaryocytic colony formation showed a linear dependence on cell dose from 0.5 to 2 .times. 105 cells/clot. The colony size frequency distribution exhibited a single peak in the 2-cell size class, followed by a continuous decrease, suggesting that cell division may occur asynchronously in cells making up a colony. Mouse [neoplastic fibroblast] L cell interferon (IF) in doses from 10-1000 U/ml was included in clots together with Ep. This resulted in a biphasic, dose-dependent reduction of colony formation and of single megakaryocyte formation. At all doses tested, CFUM were more sensitive to IF than M. These observations are best explained by a differential inhibitory effect of IF on replication of 2 or more classes of megakaryocytic progenitor cells.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 005710647

Download citation: RISBibTeXText

PMID: 6162661


Related references

Clonal growth of human megakaryocytic progenitor cells in a micro agar culture system simultaneous proliferation of megakaryocytic granulocytic and erythroid progenitor cells and t lymphocytic colonies. Experimental Hematology 11(Suppl. 14): 104, 1983

Clonal growth of human megakaryocytic progenitor cells in a micro-agar culture system: simultaneous proliferation of megakaryocytic, granulocytic, and erythroid progenitor cells (CFU-M, CFU-C, BFU-E) and T-lymphocytic colonies (CFU-TL). International Journal of Cell Cloning 1(5): 377-388, 1983

Interferon effect on megakaryocytic progenitor cells in culture. Clinical Research 27(1): 108A, 1979

Cryopreservation of human megakaryocytic progenitor cells (CFU-MK): influence of culture conditions. Cryobiology 26(3): 265-272, 1989

Alpha-interferon: differential suppression of colony growth from human erythroid, myeloid, and megakaryocytic hematopoietic progenitor cells. Journal of Interferon Research 6(3): 199-206, 1986

Improvement of culture conditions for human megakaryocytic and pluripotent progenitor cells by low oxygen tension. International Journal of Cell Cloning 5(5): 412-420, 1987

Interferon and cell division part 6 inhibitory effect of interferon on the multiplication of mouse embryo cells and mouse kidney cells in primary cultures. Proceedings of the Society for Experimental Biology & Medicine 138(3): 1044-1050, 1971

Endogenous megakaryocytic colony formation and thrombopoietin sensitivity of megakaryocytic progenitor cells are useful to distinguish between essential thrombocythemia and reactive thrombocytosis. Journal of HematoTherapy and Stem Cell Research 10(3): 405-409, 2001

In vitro differences in responsiveness of early megakaryocytic burst forming cells and late megakaryocytic colony forming units murine megakaryocyte progenitor cells. Levine, R Al (ed.). Progress In Clinical And Biological Research, Vol. 215. Megakaryocyte Development And Function; International Conference, Woods Hole, Mass., Usa, Sept. 18-21, . Xviii 435p. Alan R. Liss, Inc: New York, N.y., Usa. Illus. 179-186, 1986

Interferon and cell division. VI. Inhibitory effect of interferon on the multiplication of mouse embryo and mouse kidney cells in primary cultures. Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine 138(3): 1044-1050, 1971

Effects of amifostine on the proliferation and differentiation of megakaryocytic progenitor cells. European Journal of Pharmacology 437(1-2): 19-25, 15 February, 2002

Inhibitory effect of recombinant human tumor necrosis factor on the in vitro growth of normal human megakaryocytic progenitor cells. Journal of Cellular Biochemistry Suppl. (12 Part A): 210, 1988

Effects of leukemia inhibitory factor on the differentiation of astrocyte progenitor cells from embryonic mouse cerebral hemispheres. Developmental Brain Research. 87(2): 220-223, 1995

Expansion of marrow hematopoietic progenitor cells ex vivo supported by culture system from mouse marrow embryonic fibroblasts and leukemia inhibitory factor. Zhongguo Shi Yan Xue Ye Xue Za Zhi 12(6): 807-811, 2004

The effects of (-)-epigallocatechin-3-gallate on the proliferation and differentiation of human megakaryocytic progenitor cells. Journal of Radiation Research 47(2): 213-220, 2006