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Inorganic salts and the growth of spiroplasmas



Inorganic salts and the growth of spiroplasmas



Canadian Journal of Microbiology 32(11): 861-866



A chemically defined medium (CC-494M) was used to study the effect of inorganic salts on the growth of three spiroplasmas representing three distinct serogroups: Spiroplasma melliferum (AS 576), Spiroplasma floricola (23-6), and SR 3 spiroplasma. KH2PO4 or NaH2PO4 .cntdot. H2O was required. KH2PO4 supported faster growth and higher yield for spiroplasmas than NaH2PO4 .cntdot. H2O. The optimal concentration of KH2PO4 for S. melliferum, S. floricola, and SR 3 spiroplasma was 0.5, 0.5, and 0.25 mM, respectively, whereas that of NaH2PO4 .cntdot. H2O was 0.5, 0.05, and 2.5 mM. When supplemented with NaH2PO4 .cntdot. H2O, KCl promoted growth comparable with that obtained from KH2PO4-supplemented medium and RbCl supported limited growth, whereas NaCl, LiCl, CsCl, CaCl2, and MgSO4 .cntdot. 7H2O were inhibitory. Spiroplasma growth decreased as the concentration of LiCl and CsCl increased. At 200 mM LiCl totally inhibited the growth of S. melliferum and SR 3 spiroplasma, whereas limited growth was obtained for S. floricola. CsCl at 150 mM totally inhibited the growth of S. floricola, while limited growth was observed for S. melliferum and SR 3 spiroplasma. RbCl supported limited growth when supplemented with NaH2PO4 .cntdot. H2O whereas it was inhibitory when added in KH2PO4-supplemented medium. All the 10 potassium salts (KCH2COOH, KBr, K2CO3, KHSO4, KCl, KOH, KI, KNO3, KH2PO4, and K2HPO4) at concentrations of 20 mM promoted growth when supplemented with NaH2PO4 .cntdot. H2O.

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Accession: 005713167

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DOI: 10.1139/m86-158


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