Interconversion of mouse adult hemoglobin and a neonatal spleen fast hemoglobin band
Whitney, J.B.; McFarland, E.C.; Russell, E.S.
Developmental Biology 65(1): 233-237
ISSN/ISBN: 0012-1606 PMID: 680357 DOI: 10.1016/0012-1606(78)90193-8
Neonatal mouse spleens have, in addition to or in place of adult Hb, an electrophoretically fast Hb band. The proportion of this fast-migrating band is increased significantly by gently heating the sample prior to electrophoresis. Gentle heating of the sample in the presence of Cleland's reagent prior to electrophoresis reduces the proportion of the fast band to near zero. The fast band consists of adult-type Hb linked via a disulfide bridge to some small molecule with an overall negative charge, possibly glutathione.