EurekaMag.com logo
+ Site Statistics
References:
53,869,633
Abstracts:
29,686,251
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Interactions between octopine and nopaline plasmids in Agrobacterium tumefaciens



Interactions between octopine and nopaline plasmids in Agrobacterium tumefaciens



Journal of Bacteriology 143(3): 1295-1306



Transfer of octopine Ti plasmids to strains already carrying an octopine Ti plasmid was found to occur at the same (high) frequency as transfer to Ti plasmid lacking recipients, showing that resident Ti plasmids do not exhibit entry exclusion towards incoming Ti plasmids. The resident octopine Ti plasmid was lost by the recipient after the entrance of the incoming Ti plasmid, which is indicative of the incompatibility between the Ti plasmids. Octopine Ti plasmids were found to become established only infrequently in recipients with a nopaline Ti plasmid and, vice versa, nopaline Ti plasmids were only rarely established in recipients with an octopine Ti plasmid. Rare clones in which the incoming octopine (nopaline) Ti plasmid had been established despite the presence of a nopaline (octopine) Ti plasmid appeared to harbor cointegrates consisting of the entire incoming Ti plasmid and the entire resident Ti plasmid. The integration event invariably had occurred in a region of the plasmids that is highly conserved in evolution and that is essential for oncogenicity. These results show that octopine and nopaline Ti plasmids cannot be maintained as separate replicons by one and the same cell. Therefore, be definition, these plasmids belong to the same incompatibility group, which has been names inc Rh-1. Agrobacterial non-Ti octopine and nopaline plasmids were found to belong to another incompatibility group. The tumorigenic properties of strains harboring two different Ti plasmids, in a cointegrate structure, were indicative of the virulence genes of both of them being expressed. The agrobacterial non-Ti octopine and nopaline plasmids did not influence the virulence properties encoded by the Ti plasmid.

(PDF emailed within 1 workday: $29.90)

Accession: 005726329

Download citation: RISBibTeXText

PMID: 7410319



Related references

Plasmid dependent utilization of octopine by Agrobacterium tumefaciens harboring nopaline-type plasmids: high mutability to octopine utilizers. Scientific Papers of the College of Arts and Sciences, University of Tokyo 34(1/2): 87-96, 1984

Opine transport genes in the octopine (occ) and nopaline (noc) catabolic regions in Ti plasmids of Agrobacterium tumefaciens. Journal of Bacteriology 174(3): 841-849, 1992

Functional organization of the regions responsible for nopaline and octopine catabolism in Ti plasmids of Agrobacterium tumefaciens. Advances in molecular genetics of plant microbe interactions Volume I Proceedings of the 5th International Symposium, Interlaken, Switzerland, 9-14 September 1990: 28-31, 1991

Octopine and nopaline oxidases from Ti plasmids of Agrobacterium tumefaciens: molecular analysis, relationship, and functional characterization. Journal of Bacteriology 176(15): 4511-4517, 1994

Opine-regulated promoters and LysR-type regulators in the nopaline and octopine catabolic regions of Ti plasmids of Agrobacterium tumefaciens. Journal of Bacteriology 176(2): 495-503, 1994

Opine-regulated promoters and LysR-type regulators in the nopaline (noc) and octopine (occ) catabolic regions of Ti plasmids of Agrobacterium tumefaciens. Journal of Bacteriology 176(2): 495-503, 1994

Negative control of octopine degradation and transfer genes of octopine Ti plasmids in Agrobacterium tumefaciens. Journal of Bacteriology 139(2): 424-431, 1979

Regions of dna sequence homology between an octopine and a nopaline t i plasmid of agrobacterium tumefaciens. Molecular & General Genetics 169(2): 163-172, 1979

Octopine- and nopaline-inducible proteins in Agrobacterium tumefaciens are also induced by arginine. Current microbiology 33(3): 156-162, 1996

Arginine catabolism: a new function of both octopine and nopaline Ti-plasmids of Agrobacterium. Molecular & General Genetics 173(3): 263-269, 1979

Octopine and nopaline synthesis and breakdown genetically controlled by a plasmid of Agrobacterium tumefaciens. Molecular & General Genetics 145(2): 177-181, 1976

Size, location and polarity of T-DNA-encoded transcripts in nopaline crown gall tumors; common transcripts in octopine and nopaline tumors Agrobacterium tumefaciens. Cell 32(4): 1045-1056, 1983

Physical mapping of DNA base sequence homologies between an octopine and a nopaline Ti plasmid of Agrobacterium tumefaciens. Journal of Molecular Biology 152(2): 183-208, 1981

Sexual transmission of T-DNA in abnormal tobacco regenerants transformed by octopine and nopaline strains of Agrobacterium tumefaciens. Molecular and General Genetics 194: 500-507, 1984