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Interspecific transfer of only part of genome by fusion between nonirradiated protoplasts of nicotiana glauca and x ray irradiated protoplasts of nicotiana langsdorffii



Interspecific transfer of only part of genome by fusion between nonirradiated protoplasts of nicotiana glauca and x ray irradiated protoplasts of nicotiana langsdorffii



Japanese Journal of Genetics 58(6): 545-554



To transfer only part of genome, X-ray irradiated suspension cell protoplasts of N. langsdorffi were fused with suspension cell protoplasts of N. glauca by polyethylene glycol. Somatic hybrid calli were selected by the growth in the hormone-free medium. Some of somatic hybrid calli from fusion with irradiated protoplasts indicated the loss of small subunit polypeptide of fraction 1 protein which was coded by N. langsdorffii nuclear DNA. Cytological analysis provided information on significant decrease of chromosomes in somatic hybrid calli from fusion with irradiated protoplasts, compared with the somatic hybrid calli from fusion with non-irradiated protoplasts. In addition, isozyme analysis revealed that somatic hybrid calli from fusion with irradiated protoplasts lost particular bands of N. langsdorffii. The transfer of only part of genome from N. langsdorffii to N. glauca by fusion with X-ray irradiated protoplasts is demsonstrated.

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