Intra cellular levels of poly adenylated rna and loss of vigor in germinating wheat triticum aestivum cultivar hobbit embryos

Smith, C.A.D.; Bray, C.M.

Planta (Heidelberg) 156(5): 413-420

1982


ISSN/ISBN: 0032-0935
Accession: 005737457

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Abstract
Polyadenylated-RNA (Poly(A)+RNA) levels were studied during the germination of wheat embryos of high viability but differing vigor. In high-vigor embryos imbibed at 20.degree. C, the level of poly(A)+RNA falls dramatically over the 1st h of imbibition, then remains constant up to 3 h of imbibition before increasing rapidly to a level similar to that found in the quiescent state by 7 h of imbibition. Median-vigor embryos imbibed at 20.degree. C show similar changes in poly(A)+RNA content but the initial decrease and subsequent increase in poly(A)+RNA levels are less marked. On imbibition at 10.degree. C, the poly(A)+RNA content in high-vigor embryos decreases to a lesser extent during the 1st h than at 20.degree. C, and the level increases more slowly over the next 6 h than during the same time period at 20.degree. C. The level of poly(A)+RNA in median-vigor embryos remains constant over the first 4 h of germination and then falls to a level of .apprx. 1/2 that found in quiescent high-vigor embryos. Polyacrylamide gel electrophoresis of total-RNA samples shows that the polyadenylic acid (poly(A)) sequences occur in RNA species ranging in size from 35-7S. Polyacrylamide gel electrophoresis of isolated poly(A) sequences demonstrates the presence of 2 size classes of poly(A) in quiescent embryos, but at 20.degree. C a more heterodisperse pattern appears by 2 h of imbibition. At 10.degree. C, 2 size classes of poly(A) persist throughout the period studied in high- and median-vigor embryos, although in median-vigor embryos the ratio of larger:smaller poly(A)-tail sizes decreases more rapidly than in high-vigor embryos.