+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Ion induced release of luteinizing hormone releasing hormone thyrotropin releasing hormone and alpha melanocyte stimulating hormone from hypothalamic synaptosomes and its inhibition by vinblastine



Ion induced release of luteinizing hormone releasing hormone thyrotropin releasing hormone and alpha melanocyte stimulating hormone from hypothalamic synaptosomes and its inhibition by vinblastine



Pfluegers Archiv European Journal of Physiology 385(1): 79-84



Homogenates of rat hypothalamic tissue were fractionated by means of discontinuous sucrose density gradient centrifugation. Immunoreactive luteinizing hormone releasing hormone (luliberin, LHRH), thyrotropin releasing hormone (thyroliberin, TRH) and .alpha.-melanocyte stimulating hormone (melanotropin, .alpha.-MSH) were concentrated in the synaptosome-enriched fraction. This fraction was suspended in 0.32 M sucrose and the release of the 3 peptides was investigated. After incubation, the synaptosomes were re-isolated by ultrafiltration, and the concentration of each peptide in the ultrafiltrate was determined by radioimmunoassay. When the synaptosomal fraction was incubated at 30.degree. C in 0.32 M sucrose containing either 60 mM K+ -2 mM Ca2+ or 140 mM Na+ alone, a release of LHRH, TRH and .alpha.-MSH occurred. Of the total content, 30-50% of LHRH but only about 10% of TRH and .alpha.-MSH was releasable. When the synaptosome preparation was preincubated for 30 min at 30.degree. C with 10-4 M vinblastine, K+- as well as Na+-induced release of LHRH, TRH and .alpha.-MSH was inhibited, and the stimulatory effect of each cation was almost totally blocked by preincubation with 5 .times. 10-4 and 10-3 M vinblastine. The inhibitory action of vinblastine (5 .times. 10-4 M) did not affect the oxidation of glucose to CO2 by the synaptosomes. Synaptosome-enriched fractions of hypothalamic origin apparently are more stable with respect to LHRH, TRH and .alpha.-MSH release during incubation in isotonic sucrose than they are in ionic solutions, and the peptides are released by a vinblastine-sensitive mechanism.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 005753136

Download citation: RISBibTeXText


Related references

Release of luteinizing hormone releasing hormone thyrotropin releasing hormone and alpha melanocyte stimulating hormone from hypothalamic synaptosomes. Federation Proceedings 36(3): 321, 1977

Demonstration of a temperature dependent association of thyrotropin releasing hormone alpha melanocyte stimulating hormone and luteinizing hormone releasing hormone with sub neuronal particles in hypothalamic synaptosomes. Journal of Neurochemistry 31(5): 1125-1134, 1978

Temperature sensitive compartmentalization of thyrotropin releasing hormone luteinizing hormone releasing hormone and alpha melanocyte stimulating hormone in hypothalamic neurons. Federation Proceedings 37(3): 483, 1978

Spontaneous daily fluctuations and the effect of thyrotropin releasing hormone melanocyte stimulating hormone release inhibiting hormone and luteinizing hormone releasing hormone on growth hormone secretion during florid acromegaly. Verhandlungen der Deutschen Gesellschaft fuer Innere Medizin 81: 1521-1523, 1975

A sub cellular pool of hypo osmotically resistant particles containing thyrotropin releasing hormone alpha melanocyte stimulating hormone and luteinizing hormone releasing hormone in the rat hypothalamus. Journal of Neurochemistry 30(5): 937-948, 1978

The possible application of hypothalamic hormones thyrotropin releasing hormone follicle stimulating hormone releasing hormone luteinizing hormone releasing hormone in diagnostics and therapy in our country today our 1st experience. Diabetologia Croatica 5(1): 59-68, 1976

Inhibition of luteinizing hormone releasing hormone induced luteinizing hormone and follicle stimulating hormone release in man by synthetic competitive antagonistic luteinizing hormone releasing hormone analogs. Fertility and Sterility 29(2): 246-247, 1978

Effects of luteinizing-hormone-releasing hormone, alpha-melanocyte-stimulating hormone, naloxone, dexamethasone and indomethacin on interleukin-2-induced corticotropin-releasing factor release. Neuroimmunomodulation 2(3): 166-173, 1995

Studies using releasing factors in klinefelters syndrome responses of luteinizing hormone and follicle stimulating hormone to luteinizing hormone releasing hormone and of prolactin and thyroid stimulating hormone to thyrotropin releasing hormone before and after testosterone. Psychoneuroendocrinology 6(1): 37-44, 1981

Alpha-Melanocyte-stimulating hormone is contained in nerve terminals innervating thyrotropin-releasing hormone-synthesizing neurons in the hypothalamic paraventricular nucleus and prevents fasting-induced suppression of prothyrotropin-releasing hormone gene expression. Journal of Neuroscience 20(4): 1550-1558, 2000

Release of immuno reactive luteinizing hormone releasing hormone and thyrotropin releasing hormone from hypothalamic synaptosomes. Nature (London) 257(5524): 323-325, 1975

Effect of pre incubation with different concentrations of luteinizing hormone releasing hormone on subsequent luteinizing hormone release caused by supramaximally active amounts of luteinizing hormone releasing hormone role of luteinizing hormone releasing hormone induced protein synthesis. Life Sciences 21(11): 1621-1628, 1977

In vivo comparison of luteinizing hormone releasing hormone and follicle stimulating hormone releasing hormone activities of de glycine 10 proline 9 ethylamide luteinizing hormone releasing hormone glycine 10 proline 9 propylamide luteinizing hormone releasing hormone and luteinizing hormone releasing hormone using immature male rats. Proceedings of the Society for Experimental Biology and Medicine 146(1): 17-20, 1974

Factor c luteinizing hormone inhibitory hormone which inhibits the luteinizing hormone from basal release and from synthetic luteinizing hormone releasing hormone and studies on purification of follicle stimulating hormone releasing hormone. Biochemical and Biophysical Research Communications 63(1): 62-68, 1975

Carbon 13 nmr studies of the conformation of the x pro bond in the oligo peptide hormones thyrotropin releasing hormone luteinizing hormone releasing factor angiotensin and melanocyte stimulating hormone release inhibiting factor. Biochemical and Biophysical Research Communications 53(1): 244-250, 1973