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Iso enzyme polymorphism in st augustine grass stenotaphrum secundatum


Iso enzyme polymorphism in st augustine grass stenotaphrum secundatum



Crop Science 21(5): 778-782



ISSN/ISBN: 0011-183X

St. Augustine grasses (S. secundatum Walt. Kuntze) were investigated to determine the feasibility of their identification by isoenzyme variability. Several Florida [USA] clones of this species are morphologically indistinguishable especially when grown as turfgrass under high fertility. ADP glucose pyrophosphorylase, UDP glucose pyrophosphorylase, alcohol dehydrogenase (ADH), and acid phosphatase isoenzymes extracted from leaf tissue of 28 clones were separated by polyacrylamide disc electrophoresis. Gels of all 28 clones were stained for ADP glucose pyrophosphorylase and UDP glucose pyrophosphorylase isoenzyme activity while gels from selected clones were stained for total protein, ADH and acid phosphatase isoenzymes. Clones could be divided into several groups by quantitative differences of bands with equal electrophoretic mobility of ADP glucose and UDP glucose pyrophosphorylases. St. Augustine grasses grown in Florida could be divided into 2 groups by either ADP glucose or UDP glucose pyrophosphorylase isoenzyme systems or total protein. These groups could be further subdivided by acid phosphatase and ADH isoenzymes.

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Accession: 005759249

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