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Isolated and purified plasma and thylakoid membranes of the cyanobacterium anacystis nidulans contain immunologically cross reactive aa 3 type cytochrome oxidase


Isolated and purified plasma and thylakoid membranes of the cyanobacterium anacystis nidulans contain immunologically cross reactive aa 3 type cytochrome oxidase



Current Microbiology 14(5): 263-268



ISSN/ISBN: 0343-8651

DOI: 10.1007/bf01568134

Cell-free extracts of Anacystis nidulans were fractionated by discontinuous sucrose density gradient centrifugation resulting in the separation of two distinct types of membranes, the heavier one containing the chlorophyll and the lighter one devoid of chlorophyll. Identity of the latter with plasma membrane was confirmed by labeling of intact cells with impermeant marker, 35S-diazobenzenesulfonate, prior to cell disruption. Both membrane fractions were purified individually by repeated recentrifugation on identical gradients. Purified membranes were subjected to dissociating polyacrylamide gel electrophoresis, either type of membranes yielding a distinct polypeptide pattern. After transfer of the polypeptides to nitrocellulose by Western blotting, two of the proteins, with molecular weights of approximately 55,000 and 32,000, respectively, gave strong and specifically complementary cross-reactions with antibodies raised against subunits I and II of the aa3-type cytochrome oxidase from Paracoccus denitrificans. The findings will be discussed in terms of the presence of aa3-type cytochrome oxidase in both plasma and thylakoid membranes of Anacystis nidulans.

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Accession: 005760023

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