Isolation and characterization of 2 iso peroxidases from tobacco nicotiana tabacum cultivar xanthi tissue cultures

Kim, S.S.; Wender, S.H.; Smith, E.C.

Phytochemistry 19(2): 165-168


ISSN/ISBN: 0031-9422
Accession: 005760862

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Two isoperoxidases (Af and Cn) from the medium of tobacco tissue suspension culture WR-132 grown in darkness were purified to apparent homogeneity and partially characterized. Cn and Af have MW of about 30,000 and 54,000, respectively. Af has about 5.1% carbohydrate, but none could be detected in Cn. Both isoperoxidases appear to follow simple Michaelis-Menten kinetics with respect to guaiacol as the substrate. The Km for guaiacol are 4 and 13.3 mM for Af and Cn, respectively, while both isoperoxidases have a pH optimum at 6.5 Cn is dissimilar to other isoperoxidases from tobacco tissue cultures, but Af is very similar to isoperoxidase A3 from cv. Wisconsin-38 tobacco tissue culture.