+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Isolation and characterization of escherichia coli clones expressing cellulase genes from cellulomonas fimi



Isolation and characterization of escherichia coli clones expressing cellulase genes from cellulomonas fimi



Journal of General Microbiology 130(6): 1377-1384



A sensitive immunoassay developed previously was used to isolate a series of E. coli clones expressing cellulase genes from C. fimi. The clones fell into 3 groups. Clones in the 1st group contained plasmids with a 6.6 kb [kilobase] insert of C. fimi DNA, were strongly antigenic and contained low levels of CM-cellulase activity. Those in the 2nd group contained plasmids with a 5.0 kb insert, were weakly antigenic and contained high levels of CM-cellulase activity. Those in the 3rd group contained plasmids with a 5.6 kb insert, were weakly antigenic and contained low levels of CM-cellulase activity. Restriction analysis showed that the 3 groups carried different cellulase genes. All of these CM-cellulase activities were exported to the periplasm in E. coli, but with different efficiencies. These activities represented important components of the C. fimi cellulase complex. Their properties indicated that they were different from each other and that they probably had complementary actions.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 005762407

Download citation: RISBibTeXText

DOI: 10.1099/00221287-130-6-1377


Related references

A mutant of Escherichia coli that leaks cellulase activity encoded by cloned cellulase genes from Cellulomonas fimi. Biotechnology 2(3): 259-263, 1984

Molecular cloning of a cellulomonas fimi cellulase gene in escherichia coli. Gene 17(2): 139-146, 1982

Cloning and expression of Cellulomonas fimi β-glucosidase genes in Escherichia coli. Enzyme and Microbial Technology 11(5): 313-316, 1989

Cloning and expression of cellulomonas fimi beta glucosidase genes in escherichia coli. Enzyme & Microbial Technology 11(5): 313-316, 1989

The expression of Cellulomonas fimi cellulase genes in Brevibacterium lactofermentum. Gene 61(2): 199-206, 1987

The cellulose-binding domain (CBD(Cex)) of an exoglucanase from Cellulomonas fimi: production in Escherichia coli and characterization of the polypeptide. Biotechnology and Bioengineering 42(4): 401-409, 1993

Cellulase genes of Cellulomonas uda CB4. I. Cloning and expression of β-glucosidase genes in Escherichia coli. Journal of Biotechnology 3(4): 239-246, 1986

Molecular cloning of a Cellulomonas fimi cellulase gene in Excherichia coli Cellulolytic organisms. Gene 17(2): 139-145, 1982

Nucleotide sequence of the endoglucanase C gene (cenC) of Cellulomonas fimi, its high-level expression in Escherichia coli, and characterization of its products. Molecular Microbiology 5(5): 1221-1233, 1991

Expression of the Cellulomonas fimi cellulase genes cex and cenA from the divergent tet promoters of transposon Tn10. Archives of Microbiology 153(2): 129-133, 1990

Leakage of cellulomonas fimi cellulases from escherichia coli. FEMS Microbiology Letters 49(2): 279-283, 1988

Secretion of Cellulomonas fimi exoglucanase by Escherichia coli. Gene 44(2-3): 331-336, 1986

Cellulase genes of cellulomonas uda cb 4 ii. cloning and expression of a carboxymethylcellulose hydrolyzing enzyme endoglucanase gene in escherichia coli. Journal of Biotechnology 3(4): 247-253, 1986

Expression in Escherichia coli of the Cellulomonas fimi Structural Gene for Endoglucanase B. Applied and Environmental Microbiology 54(2): 518-523, 1988

Modulating the pH-activity profile of cellulase by substitution: replacing the general base catalyst aspartate with cysteinesulfinate in cellulase A from Cellulomonas fimi. Biochemistry 49(9): 2042-2050, 2010