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Isolation and characterization of further cis acting and trans acting regulatory elements involved in the synthesis of glucose repressible alcohol dehydrogenase in saccharomyces cerevisiae



Isolation and characterization of further cis acting and trans acting regulatory elements involved in the synthesis of glucose repressible alcohol dehydrogenase in saccharomyces cerevisiae



Molecular & General Genetics 176(3): 427-432



Starting with yeast cells lacking the constitutive alcohol dehydrogenase activity (ADHI), mutants with partially glucose-insensitive formation of ADHII were isolated. Genetic analysis showed that 4 mutants (designated ADR3c) were linked to the ADHII-structural gene, ADR2, and were cis-dominant. On derepression, 2 produced elevated ADHII-levels, indicating a promotor function of the altered controlling site. The other ADR3c-mutant alleles affected the ADHII-subunit association in diploids carrying 2 electrophoretically distinct alleles of the structural gene ADR2. Twelve semidominant constitutive mutants could be attributed to gene ADR1 (ADR1c-alleles) previously identified by recessive mutants with blocked derepression. This suggested a positive regulatory role of the ADR1 gene product on the expression of the ADHII-structural gene. A pleiotropic mutation ccr1 was epistatic over glucose-resistant ADHII-formation caused by ADR1c-alleles. From this it was concluded that CCR1 specifies for a product co-activating the structural gene or modifying the ADR1-gene product. A further regulatory element (gene designation ADR4) not linked to the structural gene could be identified upon isolation of recessive constitutive mutants adr4 from a ccr1 ADR1c-double mutant.

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