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Isolation and characterization of gamma 3 melanotropin from the neuro intermediary lobe of the rat pituitary



Isolation and characterization of gamma 3 melanotropin from the neuro intermediary lobe of the rat pituitary



Biochemical & Biophysical Research Communications 100(1): 336-343



.gamma.3-Melanotropin was isolated from the neurointermediary lobe of the rat pituitary by reversed-phase high performance liquid chromatography. Peptide mapping and amino acid analysis demonstrated that .gamma.3-melanotropin is a glycopeptide of 25 amino acids, which corresponds exactly to the .gamma.3-melanotropin sequence predicted by DNA sequencing, with the addition of an extra lysine residue at the amino terminus. The carbohydrate chain is linked to the peptide between asparagine 16 and the carboxyl terminal glutamine 25, and contains 4 mol of glucosamine/mol of peptide but no galactosamine.

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Accession: 005762481

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