Isolation and characterization of glyco peptides from digests of human tamm horsfall glyco protein
Afonso A M.M.; Charlwood, P.A.; Marshall, R.D.
Carbohydrate Research 89(2): 309-320
ISSN/ISBN: 0008-6215 Accession: 005762515
Glycopeptides were isolated from pronase digests of human Tamm-Horsfall glycoprotein and its asialo derivative. The carbohydrate moiety of the major glycopeptide preparation isolated from the former digests had an apparent MW of 4300, and those of 2 glycopeptides isolated from the latter digests had MW of 3600 and 2300. These data, together with the compositions of the glycopeptides, indicate that the Tamm-Horsfall glycoprotein has at least 5 asparagine residues substituted by complex carbohydrate moieties, 3 being of 1 type, relatively rich in galactose, and 2 containing more sialic acid but less galactose. A small amount of a mannose-rich glycopeptide was also recovered from the digests of the Tamm-Horsfall glycoprotein.