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Isolation and chemical characterization of 2 distinct link proteins from bovine nasal cartilage proteo glycan complex/



Isolation and chemical characterization of 2 distinct link proteins from bovine nasal cartilage proteo glycan complex/



Biochimica et Biophysica Acta 532(2): 242-248



Bovine nasal cartilage proteoglycan aggregates were dissociated and separated by dissociative density gradient centrifugation into proteoglycan subunits and "link fraction". The latter contained mainly the 2 "link proteins" as shown by analytical sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The 2 "link proteins" were then separated by preparative gel electrophoresis under dissociative conditions. Molecular weight and amino acid composition of both proteins are presented.

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