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Isolation and culture of protoplasts derived from embryogenic cell suspension culture of oryza sativa rice

Isolation and culture of protoplasts derived from embryogenic cell suspension culture of oryza sativa rice

Korean Journal of Botany 31(1): 41-50

Several cultivars of rice were examined for induction of embryogenic callus on a medium containing MS salts, vitamins and 2,4-D under darkness. Embryogenic callus was obtained from cultivar Cheonma with high ratio and embryo-like structures were formed from the callus on a medium with or without reduced 2,4-D. Somatic embryoids with a plumule and radicle axis surrounded by a scutellum were observed. These embryoids germinated and produced plantlets in 30 days on the same medium. Protoplasts isolated from an embryogenic cell suspension culture derived from embryogenic callus were cultured either in liquid or in agar medium and protoplast derived cell colonies were obtained in 3-4 weeks.

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