Isolation and estimation of cyto kinins and cyto kinin containing transfer rna from cucumis sativus cultivar guntur seedlings

Jayabaskaran, C.; Jacob, T.M.

Plant Physiology (Rockville) 70(5): 1396-1400


ISSN/ISBN: 0032-0889
Accession: 005764437

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N6-(.DELTA.2-Isopentenyl) adenosine antibodies were used for the isolation of free cytokinins and cytokinin-containing tRNA from parts of C. sativus L. cv.-Guntur seedlings and for the estimation of cytokinins in them. Immobilized N6-(.DELTA.2-isopentenyl) adenosine antibodies retained tRNA containing N6-(.DELTA.2-isopentenyl) adenosine and N6-(4-hydroxy-3-methylbut-2-enyl) adenosine with equal efficiencies. There were at least 5 cytokinins in the free form in cucumber seedlings. N6-(4-Hydroxy-3-methylbut-2-enyl) adenosine, N6-(.DELTA.2-isopentenyl) adenosine and N6-(.DELTA.2-isopentenyl) adenine were present at least to the extent of 80, 23 and 9 ng, respectively, in the cotyledons and 40, 6 and 3 ng, respectively, in the decotyledonated seedlings/g of tissue. Only 2 cytokinins were present in the tRNA of cucumber cotyledons, N6-(.DELTA.2-isopentenyl) adenosine and N6-(4-hydroxy-3-methylbut-2-enyl) adenosine in amounts of 12 and 318 ng, respectively, per gram of tissue. Immunoaffinity chromatographic analysis of radiolabeled aminoacyl tRNA from cucumber cotyledons showed that tRNAPhe and tRNATyr contained cytokinins whereas tRNAAla did not.