Isolation and partial characterization of the protein body membrane from mature and germinating seeds of vicia faba

Weber, E.; Suess K H.; Neumann, D.; Manteuffel, R.

Biochemie und Physiologie der Pflanzen (BPP) 174(2): 139-150


Accession: 005765744

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EM studies of V. faba seess showed that the protein body envelope is a protein-lipid membrane with a mean diameter of about 100 .ANG. Protein body membranes from V. faba seeds were separated from protein body contents by successive salt extractions after sonication of the isolated protein bodies. Purity of the remaining membrane fraction was checked by serological techniques. A protein content of about 20% was determined on a dry weight basis. Polyacrylamide gel electrophoresis in the presence of SDS revealed about 30 polypeptides with apparent MW up to 85,000 dalton. Among them were 15 polypeptides which showed a positive reaction after Schiff's staining. After urea treatments of the membrane fraction differences in the gel electropherograms of urea extractable polypeptides compared with the urea treated material were observed. SDS-polyacrylamide gel electrophoresis of protein body membranes from germinating seeds revealed no detectable changes with respect to main polypeptides of the membrane fraction during the first 6 days of seed germination. The presence of a membrane boundary was demonstrated by EM studies of protein bodies in the cotyledons from germinating seeds even after partial loss of contrasted material from the protein body contents.