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Isolation and purification of bio polymers by affinity chromatography 5. affinity chromatography of pyruvate decarboxylase from brewers yeast saccharomyces carlsbergensis


Isolation and purification of bio polymers by affinity chromatography 5. affinity chromatography of pyruvate decarboxylase from brewers yeast saccharomyces carlsbergensis



Bioorganicheskaya Khimiya 6(10): 1572-1579



ISSN/ISBN: 0360-4497

New biospecific adsorbents containing thiamine, thiamine pyrophosphate or imidazole as ligands and distinguished by various degrees of hydrophobicity were prepared. The pyruvate decarboxylase behavior on these adsorbents was studied for elucidating the contribution of non-specific and biospecific interaction to enzyme adsorption. A method for isolation of electrophoretically homogeneous 310-fold pyruvate decarboxylase from brewer's yeast in 14% overall yield was developed. It involves extraction by glycerol-EDTA solution, acetone and (NH4)2SO4 fractionation, Pb(CH3COO)2 treatment, gel filtration of Sephadex G-25 and affinity chromatography on thiamine pyrophosphate-4-azobenzyl-.epsilon.-aminocaproyl-hydrozido-Sepharose.

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Accession: 005766434

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Related references

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