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Isolation and purification of bio polymers by affinity chromatography part 1 new highly effective biospecific adsorbents for bio affinity chromatography of poly adenylated messenger rna


Isolation and purification of bio polymers by affinity chromatography part 1 new highly effective biospecific adsorbents for bio affinity chromatography of poly adenylated messenger rna



Bioorganicheskaya Khimiya 5(1): 92-99



ISSN/ISBN: 0360-4497

New, highly effective, biospecific adsorbents for affinity chromatography of poly(A)-RNA were prepared: poly(U)-aminoethylcarbamoyldextran-Sepharose (adsorbent A) and poly(U)-glycogen-hydrazidosuccinyl-Sepharose (adsorbent B). The poly(U) content was 1.47 and 1.45 mg/ml, and poly(A) binding capacity was 1.44 and 1.48 mg/ml for the adsorbents A and B, respectively. The chromatographic studies of poly(A) and poly(U) using these adsorbents demonstrated the electrostatic nature of non-specific polynucleotide-adsorbent binding. Isolation of poly(A)-mRNA from mouse liver cells was performed on adsorbent B, which is the first example of application of biospecific adsorbents with polysaccharide spacers for affinity chromatography. Optimal conditions for poly(A)-mRNA affinity chromatography, aimed at obtaining the biopolymer in a quantitative yield, are discussed.

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Related references

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