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Isolation of antigenic variants from leptospiras grown in vitro and from heart blood of guinea pigs inoculated with a cloned strain of leptospira



Isolation of antigenic variants from leptospiras grown in vitro and from heart blood of guinea pigs inoculated with a cloned strain of leptospira



Zentralblatt fuer Bakteriologie Parasitenkunde Infektionskrankheiten und Hygiene Erste Abteilung Originale Reihe A Medizinische Mikrobiologie und Parasitologie 245(3): 345-355



A cloned culture of L. interrogans serovar copenhageni strain Shibaura (Cl-Shibaura) was inoculated into guinea pigs. The heart blood of the guinea pigs, obtained at the febrile stage and inoculated onto a solid serum medium containing homologous immune serum, produced large and small colonies. Serological examinations revealed that the large colonies were mainly antigenic variants; small colonies were mostly the parent. Antigenic variants accounted for 16.4% of all colonies from the blood of guinea pigs infected with Cl-Shibaura and for 1.2% of all colonies from the culture of Cl-Shibaura in normal serum medium. Antigenic variants were also isolated in vitro from the culture of 2 other serovars. The fact that the frequency of antigenic variants of leptospiras was higher in vivo than in vitro is discussed.

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Accession: 005769177

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PMID: 44789


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