Isolation of large and small granules of barley starch and a study of factors influencing the adsorption of barley malt alpha amylase by these granules
Cereal Chemistry 56(5): 430-434
ISSN/ISBN: 0009-0352 Accession: 005770814
An elutriation column was used to separate barley starch granules into a prime starch fraction and a secondary starch fraction with mean particle diameters of 16.7 and 1.87 .mu.m, respectively. By microscopic examination, each fraction was relatively homogeneous. Secondary starch, presumably because of its greater available surface area, was more efficient than prime starch in adsorbing malt .alpha.-amylase from solution. Adsorption was greatest in the pH range 5-6 and was 7 times higher at 5.degree. C than at 28.degree. C.