Isozyme characteristics of glucose 6 phosphate dehydrogenase and lactate dehydrogenase of continuous cells from the collection of the sverdlovsk research institute of viral infections
Tsareva, A.A.; Glinskikh, N.P.; Dreizin, R.S.; Kolesnikova, G.G.; Zhdanov, V.M.
Voprosy Virusologii 5: 620-623
ISSN/ISBN: 0507-4088 Accession: 005774309
An analysis of glucose-6-phosphate dehydrogenase (G-6-P-DH) and lactate dehydrogenase (LDH) isozymes of established cell lines from the collection of Sverdlovsk Research Institute of Virus Infections was carried out. Continuous human and animal cell lines  were examined by electrophoresis in a vertical block of 7% polyacrylamide gel followed by histochemical detection of the enzymes. HeP-2 [human laryngeal carcinoma], HEp-2 clone 28, KB [human oral epidermoid carcinoma], FL [human amnion?] and RH [human embryonic kidney] apparently had the electrophoretic motility of G-6-PDG characteristic of HeLa [human cervical carcinoma] cells. LEP [human embryonic lung] and SOC cell lines were contaminated with mouse cells. A culture of rat fibroblasts had isoenzymes of G-6-PDG and LDH characteristic of HeLa cells. Remaining cell lines had isoenzymatic characteristics corresponding to their species appurtenance.