Section 6
Chapter 5,775

Isozyme characteristics of glucose 6 phosphate dehydrogenase and lactate dehydrogenase of continuous cells from the collection of the sverdlovsk research institute of viral infections

Tsareva, A.A.; Glinskikh, N.P.; Dreizin, R.S.; Kolesnikova, G.G.; Zhdanov, V.M.

Voprosy Virusologii 5: 620-623


ISSN/ISBN: 0507-4088
Accession: 005774309

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An analysis of glucose-6-phosphate dehydrogenase (G-6-P-DH) and lactate dehydrogenase (LDH) isozymes of established cell lines from the collection of Sverdlovsk Research Institute of Virus Infections was carried out. Continuous human and animal cell lines [17] were examined by electrophoresis in a vertical block of 7% polyacrylamide gel followed by histochemical detection of the enzymes. HeP-2 [human laryngeal carcinoma], HEp-2 clone 28, KB [human oral epidermoid carcinoma], FL [human amnion?] and RH [human embryonic kidney] apparently had the electrophoretic motility of G-6-PDG characteristic of HeLa [human cervical carcinoma] cells. LEP [human embryonic lung] and SOC cell lines were contaminated with mouse cells. A culture of rat fibroblasts had isoenzymes of G-6-PDG and LDH characteristic of HeLa cells. Remaining cell lines had isoenzymatic characteristics corresponding to their species appurtenance.

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