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Labeling of sarcoma associated monoclonal antibody with indium 111 gallium 67 and iodine 125

Labeling of sarcoma associated monoclonal antibody with indium 111 gallium 67 and iodine 125

Radiotherapy & Oncology 8(2): 129-136

Labeling of human sarcoma-associated murine monoclonal antibody (MAb) 23H7 with 67Ga and 111In by the bifunctional ligand method is reported. 67Ga was chelated to the MAb via desferrioxamine B and 111In via the cyclic anhydride of DTPA. Higher specific activity was obtained with 67Ga (4-5 .mu.Ci/.mu.g) as compared with 111In (2 .mu.Ci/.mu.g). The binding capacity of the MAb was confirmed by repeated indirect immuno-fluorescence assays performed before and after labeling. A fast blood clearance was observed: 33% recovered dose (R.D.) blood level 3 h post-injection as compared with 56% after injection of control polyclonal IgG. Preliminary results on chemically induced sarcoma bearing mice showed a relatively high tumor uptake of the labeled antibody.

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