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Labeling of some plant viruses with carbon 14



Labeling of some plant viruses with carbon 14



Eesti NSV Teaduste Akadeemia Toimetised Bioloogia 26(1): 66-68



When studying the biosynthesis of virus particles (translation of virus RNA in extracellular protein-synthesizing systems, self-assembly of virus particles from nucleic acid and from structural protein) and the physico-chemical properties of virions, it is essential to have labeled radioactive isotope (14C, 3H, 32P) preparations of the viruses and their components. A method is described for obtaining 14C labeled tobacco mosaic viruses, X23 viruses and potato NR (BX23K, BNRK) viruses from leaves of Nicotiana tabacum and N. glutinosa by saturation of the leaves with virus preparations followed by inoculation of a 14C labeled amino acid preparation after several days when the virion concentrations reached their maximum levels. Labeled preparations were obtained with specific activity of 0.29 (tobacco mosaic virus), 0.14 (X23) and 0.03 (potato NR) .mu.Ci to 1 mg of structural protein of the virus.

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