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Labeling of the active subunit of cholera toxin from within the membrane bi layer



Labeling of the active subunit of cholera toxin from within the membrane bi layer



Biochemical & Biophysical Research Communications 87(1): 308-313



Using the photoreactive glycolipid probe 12-(4-azido-2-nitrophenoxy)-stearoylglucosamine-[1-14C], the active A1 subunit of cholera toxin was radiolabeled from within the membrane bilayer. The membrane employed as a target was the envelope of Newcastle disease virus which contained the photoreactive probe. Radiolabeling of the A1 subunit occurred after cholera toxin and virus were incubated together for 15 min at 37.degree. C and then irradiated at 366 nm for 1 min. Labeling of A1 did not occur when cholera toxin was irradiated in a solution of probe without virus or when the 15 min incubation with virus was performed at 0.degree. C instead of at 37.degree. C.

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Accession: 005787071

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PMID: 454405


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