+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Labeling of the active subunit of cholera toxin from within the membrane bi layer

Labeling of the active subunit of cholera toxin from within the membrane bi layer

Biochemical & Biophysical Research Communications 87(1): 308-313

Using the photoreactive glycolipid probe 12-(4-azido-2-nitrophenoxy)-stearoylglucosamine-[1-14C], the active A1 subunit of cholera toxin was radiolabeled from within the membrane bilayer. The membrane employed as a target was the envelope of Newcastle disease virus which contained the photoreactive probe. Radiolabeling of the A1 subunit occurred after cholera toxin and virus were incubated together for 15 min at 37.degree. C and then irradiated at 366 nm for 1 min. Labeling of A1 did not occur when cholera toxin was irradiated in a solution of probe without virus or when the 15 min incubation with virus was performed at 0.degree. C instead of at 37.degree. C.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 005787071

Download citation: RISBibTeXText

PMID: 454405

Related references

The effect of cholera toxin and cholera toxin B subunit on the nasal mucosal membrane. Vaccine 9(11): 825-832, 1991

Synergistic action of cholera toxin B subunit (and Escherichia coli heat-labile toxin B subunit) and a trace amount of cholera whole toxin as an adjuvant for nasal influenza vaccine. Vaccine 12(5): 419-426, 1994

Cautionary note on the use of the B subunit of cholera toxin as a ganglioside GM1 probe: detection of cholera toxin A subunit in B subunit preparations by a sensitive adenylate cyclase assay. Journal of Cellular Biochemistry 42(3): 143-152, 1990

The adjuvant effect of cholera toxin cholera toxin b subunit and glutaraldehyde modified cholera toxin on the mucosal immune response of rabbits to 2 acetylaminofluorene thyroglobulin conjugates. FASEB Journal 4(3): A500, 1990

Labeling of neuronal connections with monoclonal antibodies against cholera toxin subunit b. Neuroscience Letters Suppl. (22): S204, 1985

A promoter responsible for over-expression of cholera toxin B subunit in cholera toxin A subunit structure gene. Yi Chuan Xue Bao 24(1): 78-86, 1997

Simultaneous anterograde labeling of different afferent pathways using biotinylated dextran amine and cholera toxin subunit B. Society for Neuroscience Abstracts 19(1-3): 1115, 1993

Amygdaloid projections to the ventromedial nucleus of hypothalamus A retrograde labeling study using cholera toxin b subunit. Society for Neuroscience Abstracts 19(1-3): 1441, 1993

Differential labeling of converging afferent pathways using biotinylated dextran amine and cholera toxin subunit B. Journal Of Neuroscience Methods. 52(2): 143-148, 1994

Photo labeling of cholera toxin subunits during membrane penetration. Nature (London) 289(5795): 319-321, 1981

Fragmentation and purification of cholera toxin active subunit. Zentralblatt fuer Bakteriologie Suppl. 19: 79-80, 1990

Double labeling method using fluorescent labeled cholera toxin B subunit for the purpose of analysis of neural structures. No to Hattatsu 27(3): 253-254, 1995