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Labeling of the intra membranous region of the major sialo glyco protein of human erythrocytes with a photo sensitive hydrophobic probe



Labeling of the intra membranous region of the major sialo glyco protein of human erythrocytes with a photo sensitive hydrophobic probe



Biochemical Journal 179(2): 265-272



Human erythrocyte membranes were incubated with the photosensitive hydrophobic reagent 1-azido-4-iodo[3H]benzene and the mixture was irradiated. The major sialoglycoprotein was then isolated and the labeled polypeptide was subjected to proteolytic dissection. Characterization of the purified tryptic and chymotryptic peptides showed that the probe was covalently attached only to the transmembrane region of the protein. This labeling pattern was discussed in relation to the use of such reagents for the identification of segments of membrane proteins exposed to the hydrophogic miliue of the membrane.

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Accession: 005787080

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PMID: 486078


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