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Labeling of the rat spermatid nucleus with tritiated arginine

Labeling of the rat spermatid nucleus with tritiated arginine

Hereditas (Lund) 102(1): 63-70

The incorporation of 3H-Arg by the nucleus of the rat spermatid was studied with EM autoradiography. The nucleus is slightly labeled with 3H-Arg until step 9 of spermiogenesis, where the labeling ceases when the nucleus condenses. Thereafter there is a high level of 3H-Arg incorporation into the spermatid nuclei at steps 15-16, reflecting the formation of the Arg rich sperm histone. This histone is synthesized in the cytoplasm close to the nuclear membrane. Evidently the Arg rich sperm histone does not play any role in the condensation of the sperm nuceli or in the cessation of RNA synthesis. It appears to be the package form of the DNA during the period of its transit from one generation to the next.

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