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Labeling of the thymidine and deoxy cytidine bases of dna by carbon 14 labeled deoxy cytidine in cultured l 1210 cells

Labeling of the thymidine and deoxy cytidine bases of dna by carbon 14 labeled deoxy cytidine in cultured l 1210 cells

Cancer Letters 19(2): 147-158

Exposure of cultured mouse leukmeia L1210 cells to [2-14C]deoxycytidine and analysis of radioactivity incorporated into DNA-pyrimidines revealed that 2.7-to 5.5-fold more radioactivity is incorporated into DNA-thymine than into cytosine bases. Thus the pathway involving deamination of deoxycytidylate to deoxyuridylate and methylation to thymidylate is highly favored over successive phosphorylation to dCTP. Several modified and endogenous pyrimidines altered the labeling of DNA-thymine and DNA-cytosine with [2-14C]-deoxycytidine. 3-Deazauridine at 0.1 mM caused a 56% increase in the labeling of DNA-thymine. Both thymidine and 3-deazauridine (.gtoreq. 10 .mu.M) increased the specific activity of DNA-cytosine by 4-fold. Cytosine arabinoside (ara-C) (.gtoreq. 10.mu.m) reduced the labeling of both DNA-cytosine and DNA-thymine. Excess cytidine (0.1 mM) reduced the labeling of DNA-cytosine by 40%. Tetrahydrouridine at concentrations up to 1 mM had no effect. [Deoxcytidine modifies the toxic effects of several antineosplastic drugs].

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