Labeling pattern of the nonhistone chromosomal proteins in quiescent and dividing ehrlich ascites tumor cells
Labeling pattern of the nonhistone chromosomal proteins in quiescent and dividing ehrlich ascites tumor cells
Anachkova, B.; Russev, G.
International Journal of Biochemistry 15(8): 1093-1094
1983
Upon pulse-labeling with [14C]protein hydrolizate both in dividing and quiescent mouse Ehrlich ascites tumor (EAT) cells the nonhistone chromosomal (NHC) proteins had uniform specific radioactivity with few exceptions: Both in quiescent and dividing EAT cells a polypeptide with MW of .apprx. 200 kdalton had specific radioactivity 2-3 times lower than that of the most of the NHC proteins. In the actively proliferating cells a group of proteins with MW between 45 and 65 kdalton had 2-3 times higher specific radioactivity than most of the NHC proteins. In quiescent cells the specific radioactivity of a group of proteins with MW in the range 18-25 kdalton was 3-4 times higher than that of the rest of the NHC proteins.