+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Labeling plasmin with technetium 99m for scintigraphic localization of thrombi



Labeling plasmin with technetium 99m for scintigraphic localization of thrombi



International Journal of Applied Radiation and Isotopes 28(1-2): 97-104



A detailed study was made of the method for labeling plasmin with 99mTc in order to prepare a radioactive indicator for early scintigraphic visualization of thrombi and tumors. The best method found for preparing 99mTc-plasmin involved the reduction of 2.5 ml 99mTc-pertechnetate solution with 0.5 ml of 4 mM SnCl2, 2 M NaCl and 70 mM HCl. This mixture was then added to 5 mg of plasmin to give a final pH of about 2. After 60 min of reaction the labeling efficiency was 80-90% as determined by gel chromatography column-scanning. The labeling kinetics and influence of pH, concentration of SnCl2, NaCl, plasmin and lysine were studied. The enzymatic activity of plasmin was reduced by less than 15% by the labeling process. Preliminary experiments in rabbits with artificially induced thrombi indicate accumulation of 99mTc activity in the same area as 125I-fibrinogen after the administration of 99mTc-plasmin.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 005787099

Download citation: RISBibTeXText

PMID: 140151


Related references

Demonstration of technetium 99m labeled plasmin on the surface of ex vivo thrombi. Thrombosis Research 28(6): 783-792, 1982

Preparation and testing of technetium 99m labeled strepto kinase for scintigraphic imaging of thrombi. Journal Of Nuclear Medicine: 557, 1975

An in vitro model for the scintigraphic detection of thrombi using a technetium 99m labelled antifibrin monoclonal antibody. Nuclear Medicine Communications 10(9): 653-660, 1989

Platelet labeling with indium 111 labeled oxine 2. scintigraphic detection of thrombi in man. Kaku Igaku 18(5): 715, 1981

Labeling of plasmin with technetium 99m. Journal of Labelled Compounds & Radiopharmaceuticals 13(2): 167, 1977

Quality control of technetium 99m plasmin preparations for scintigraphic detection of deep venous thrombosis. Journal of Labelled Compounds & Radiopharmaceuticals 16(1): 201, 1979

Contact thermography technetium 99m plasmin scintimetry and technetium 99m plasmin scintigraphy as screening methods for deep venous thrombosis following major hip surgery. Thrombosis & Haemostasis 58(3): 831-833, 1987

Labeling of epirubicin with technetium-99m: Optimization, biodistribution and scintigraphic imaging in tumor bearing mice. Pakistan Journal of Pharmaceutical Sciences 31(2): 517-524, 2018

Scintigraphic localization of the placenta using technetium, Tc 99m tagged albumin. Fortschritte auf dem Gebiete der Rontgenstrahlen und der Nuklearmedizin 106(3): 381-386, 1967

Scintigraphic localization of tumors by use of technetium 99m labelled biotin as a tracer. Journal of Cancer Research & Clinical Oncology 116(SUPPL PART 1): 194, 1990

Anti-CEA monoclonal antibody: technetium-99m labeling and the validation process of a scintigraphic animal model with a non-cellular antigenic implant. Cellular and Molecular Biology 48(5): 511-516, 2002

Giant cell arteritis scintigraphic localization using technetium-99m S12 Fab monoclonal antibody. Journal of Nuclear Medicine 37(5 SUPPL ): 242P, 1996

Localization of epileptogenic foci contribution of scintigraphic tomography using technetium 99m hmpao. Clinical & Investigative Medicine 11(4 SUPPL): C92, 1988

Scintigraphic localization of pulmonary bleeding using technetium Tc 99m sulfur colloid: a preliminary report. Radiology 143(3): 757-762, 1982

Limitations of technetium 99m sestamibi scintigraphic localization for primary hyperparathyroidism associated with multiglandular disease. American Surgeon 69(2): 170-175, 2003