+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Labeling platelets with radioactive chromium 51 for platelet antibody detection



Labeling platelets with radioactive chromium 51 for platelet antibody detection



Journal of the American Medical Technologists 39(2): 74-78



A technique for the use of radioactive Cr51 for the detection of [human] platelet antibodies is proposed. Results of a series of procedural variations such as amounts, times and temperatures, agree with other authors. An evaluation of the data shows that for routine use, the methods now available appear to be as effective as the Cr51 method. The proposed method is more time consuming than the platelet complement fixation (PCF) procedure; another important aspect is the need for fresh cells, not more than 1 day old. There is also the apparent requirement for the presence of a strong antibody. The incubation of the platelet-chromate mixture should be in as small a volume as possible. Saline should be used as the suspension media. The mixture should be incubated at 37.degree. C for 40 min.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 005787100

Download citation: RISBibTeXText


Related references

Storage of reagent platelets for anti platelet antibody testing in the chromium 51 platelet lysis assay. Journal of Clinical Pathology 32(2): 191-192, 1979

Biosynthetic labeling of platelet activating factor from radioactive acetate by stimulated platelets. Nature (London) 289(5795): 312-314, 1981

Cryo preservation of human platelets chromium 51 labeling characteristics of fresh and frozen platelets. Cryobiology 14(6): 699, 1977

Platelet kinetics with indium-111 platelets: comparison with chromium-51 platelets. Seminars in Thrombosis and Hemostasis 9(2): 100-114, 1983

Ultrastructural localization of platelet factor 4 in rat megakaryocytes and platelets by gold-labeled antibody detection. Experimental Hematology 17(3): 223-228, 1989

Aspirin inhibits platelet aggregation on but not adhesion to collagen fibrils an assessment of platelet adhesion and deposited platelet mass by morphometry and chromium 51 labeling. Thrombosis Research 11(5): 619-632, 1977

The use of radioactive chromium-51 for labeling bacteria. Bull Vet Inst Pulawy 10(3/4): 152-156, 1966

AET-treated platelets: their usefulness for platelet antibody detection and an examination of their altered sensitivity to immune lysis. Blood 54(5): 1101-1108, 1979

Indium 111 and chromium 51 labeling of platelets are they comparable. Transfusion 26(1): 16-19, 1986

Detection of activated platelets in urinary sediments by immunofluorescence using monoclonal antibody to human platelet GMP-140 in patients with IgA nephropathy. Journal Of Clinical Laboratory Analysis. 7(6): 329-333, 1993

Platelet antibody detection using frozen pooled human lymphocyte antigen-typed platelets as target cells. Diagnostic and Clinical Immunology 5(6): 377-380, 1988

Indium-111 (111 In) and chromium-51 (51Cr) labeling of platelets. Are they comparable?. Transfusion 26(1): 16-19, 1986

The Detection of Platelet-Bound Immunoglobulin Using Antibody-Coated Polyacrylamide Beads and Paraformaldehyde-Stabilized Platelets . . . A Feasibility Study. Laboratory Medicine 10(8): 486-489, 1979

Technological labeling of erythrocytes by radioactive chromium Abstr. only. Lab Delo 2: 124-125, 1967

Labeling of thymocytes using radioactive chromium Cr-51. II. Principles and technic. Polski Przeglad Radiologii i Medycyny Nuklearnej 30(4): 403-409, 1966