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Labeling platelets with radioactive chromium 51 for platelet antibody detection

Labeling platelets with radioactive chromium 51 for platelet antibody detection

Journal of the American Medical Technologists 39(2): 74-78

A technique for the use of radioactive Cr51 for the detection of [human] platelet antibodies is proposed. Results of a series of procedural variations such as amounts, times and temperatures, agree with other authors. An evaluation of the data shows that for routine use, the methods now available appear to be as effective as the Cr51 method. The proposed method is more time consuming than the platelet complement fixation (PCF) procedure; another important aspect is the need for fresh cells, not more than 1 day old. There is also the apparent requirement for the presence of a strong antibody. The incubation of the platelet-chromate mixture should be in as small a volume as possible. Saline should be used as the suspension media. The mixture should be incubated at 37.degree. C for 40 min.

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Accession: 005787100

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