+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Labelling of some enzymes by electrolytic iodination a comparative study

Labelling of some enzymes by electrolytic iodination a comparative study

Journal of Radioanalytical & Nuclear Chemistry 102(2): 483-492

The effect of the degree of iodination on the enzymatic activity of two enzymes, phospholipase A2 (fraction k1) and cathepsin D, was studied. The compounds were labelled with 131T3 and .**GRAPHIC**. prepared by electrolysis at a controlled electrode potential. To avoid excessive denaturation of the enzyme, the electrophilic reagent was produced separately and added to the enzyme solution. Unreacted iodine species were removed by means of gel chromatography on Sephadex G-25. Enzymes were also radioiodinated by a chemical method, using chloramine T as oxidant, the results being compared with those obtained by the electrochemical method.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 005787126

Download citation: RISBibTeXText

DOI: 10.1007/bf02047922

Related references

Labelling Of Human Fibrinogen With 131-I By Electrolytic Iodination. Biochimica et Biophysica Acta 86: 519-526, 1964

Electrolytic iodination of a tetracycline. International Journal of Nuclear Medicine & Biology 9(3): 225-226, 1982

Electrolytic iodination of proteins with I-125 and I-131. Journal of Laboratory and Clinical Medicine 72(6): 966-970, 1968

Micro electrolytic iodination of poly peptide hormones. International Journal of Applied Radiation & Isotopes 30(6): 359-364, 1979

Electrolytic iodination of Fasciola hepatica antigen with 125I. Acta Veterinaria 50(1/2): 101-103, 1981

An electrolytic procedure for iodination of glycoprotein and protein hormones. Journal of Endocrinology 61(2): Xlii-Xlii, 1974

An effective electrolytic iodination of human serum albumin at alkaline pH. International Journal of Applied Radiation and Isotopes 23(5): 242-244, 1972

Micro-electrolytic iodination of polypeptide hormones for radio-immunoassay. Clinica Chimica Acta; International Journal of Clinical Chemistry 36(2): 517-520, 1972

Optimization of thyroid stimulating hormone iodination comparison of enzymatic electrolytic and chloramine t methods. Clinical Chemistry 25(6): 1065, 1979

Micro electrolytic radio iodination of poly peptide hormones to high specific activity. International Journal of Applied Radiation and Isotopes 29(9-10): 567-570, 1978

Labelling of proteins by use of iodination and detection by ICP-MS. Journal Of Analytical Atomic Spectrometry: 11, 1487-1496, 2008

Labelling of DNA by iodination and its application on the detection of EBV-DNA. Zhonghua Zhong Liu Za Zhi 8(2): 107-111, 1986

Protein Labelling With Radioiodine By Electrochemical Way. I. Iodination Of Tyrosine. La Ricerca Scientifica. 2. Ser., Pt. 2: Rendiconti. Sezione B: Biologica 18: 669-680, 1964

About insulin iodination. II. Comparative study of enzymic and chemical methods. Biochimie 56(9): 1191-1196, 1974