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Lability of coupling between proton translocase and atpase of mitochondrial proton atpase complex

Lability of coupling between proton translocase and atpase of mitochondrial proton atpase complex

Biokhimiya 44(6): 975-982

The interrelationship between the ATPase and H+-translocase functions of mitochondrial H+-ATPase was studied. The efficiency of the functioning was estimated by the value of coupling coefficient (Kc), which is represented by a ratio of proton translocation rate vs. ATP coupling hydrolysis rate. Under conditions of increased concentrations of ATP and low concentrations of oligomycin the value of Kc is decreased. The increase in the concentration of valinomycin results in an increase of Kc. The H+-ATPase activity increases considerably during incubation of mitochondria, reaching its maximum with respect to both functions 1-2 min after addition of ATP. A lack of tight coupling between the H+-translocase and ATPase functions of mitochondrial H+-ATPase S indicated. The mechanism of action of H+-ATPase is discussed.

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