+ Site Statistics
References:
52,572,879
Abstracts:
28,705,754
PMIDs:
27,750,366
DOIs:
25,464,004
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Labilization of cerebral lysosomes by autologous microsomes and protection by cytosolic fraction and a super oxide scavenger in vitro



Labilization of cerebral lysosomes by autologous microsomes and protection by cytosolic fraction and a super oxide scavenger in vitro



Biochemical & Biophysical Research Communications 86(4): 1222-1226



Incubation of rat cerebral lysosomes with autologous microsomes resulted in labilization of the lysosomal complex as assayed through determination of lysosomal acid proteinase activity in the incubate supernatant. Addition of cytosolic fraction with significant glutathione peroxidase activity largely prevented the labilization whereas an artificial scavenger of active O2 (benzoic acid) was not as effective. Frozen and thawed lysosomes served as controls. The labilization of lysosomes by microsomal O2 activation mechanism in the absence of exogenous substrate may explain neuronal degeneration in cases of induction of the oxidative activity without generation of reactive metabolites from the exogenous substrate.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 005787243

Download citation: RISBibTeXText

PMID: 435321


Related references

Studies on gentamicin-induced labilization of rat kidney lysosomes in vitro. Possible protection by selenium. Biochemical Pharmacology 32(18): 2659-2664, 1983

Cerebral lysosomes. 3. Evidence for in vivo "labilization" by the convulsant DL-methionine-DL-sulfoximine. Life Sciences 5(2): 163-167, 1966

Labilization of brain lysosomes by trichloroethylene metabolites and peroxide anion in vitro. Research Communications in Chemical Pathology and Pharmacology 19(3): 533-536, 1978

Mechanism of "in vitro" labilization of lysosomes from rat liver and kidney by mercuric chloride. Archives Internationales de Physiologie et de Biochimie 79(3): 621-622, 1971

Comparative study of the labilization of lysosomes peroxisomes and mitochondria by inorganic mercury in vitro. Reid, Eric (Ed ) Methodological Developments in Biochemistry, Vol 4 Subcellular Studies Symposium Guildford, England, July 12-14, 1973 Xii+438p Illus Longman Inc: New York, N Y , U S A ; London, England ISBN 0-582-46026-3: 167-175, 1974

Stabilization of hepatic lysosomes of rats by vitamin E and selenium in vivo as indicated by thermal labilization of isolated lysosomes. Journal of Nutrition 102(11): 1413-1419, 1972

Influence of vitamin A acetate, alcohol and their radiolytic products on the in vitro labilization of lysosomes and tissue autolysis. Acta Vitaminologica et Enzymologica 26(5): 181-188, 1972

In vitro biotransformation of 3,4-dihydro-6-hydroxy-2,2-dimethyl-7-methoxy-1 -benzopyran , a potent lipid peroxidation inhibitor and nitric oxide scavenger, in rat liver microsomes. Chemical Research in Toxicology 17(7): 904-913, 2004

Mechanism of action of miconazole: labilization of rat liver lysosomes in vitro by miconazole. Antimicrobial Agents and ChemoTherapy 9(6): 903-907, 1976

Lipid per oxide formation in epidermal microsomes studies on the role of super oxide hydrogen per oxide and singlet oxygen. Journal of Investigative Dermatology 78(4): 352, 1982

Chlordecone increased subcellular distribution of scavenger receptor class B type II to murine hepatic microsomes without altering cytosolic cholesterol binding proteins. Toxicology Letters 191(1): 20-25, 2009

Role of cyclic amp and a cytosolic fraction in the calcium uptake by rat liver microsomes. Biochemical Society Transactions 9(2): 140P, 1981

Solvent exchange in AlF (sub x) (H (sub 2) O) (sub 6-x) (super 3-x) (aq) complexes; ligand-directed labilization of water as an analogue for ligand-induced dissolution of oxide minerals. Geochimica et Cosmochimica Acta 61(15): 3041-3049, 1997

Labilization and Stabilization of Lysosomes. Federation Proceedings 23: 1038-1044, 1964

Is ascorbate a super oxide radical scavenger?. Archivos de Biologia y Medicina Experimentales 14(2): 203, 1981