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Labilization of cerebral lysosomes by autologous microsomes and protection by cytosolic fraction and a super oxide scavenger in vitro

Labilization of cerebral lysosomes by autologous microsomes and protection by cytosolic fraction and a super oxide scavenger in vitro

Biochemical & Biophysical Research Communications 86(4): 1222-1226

Incubation of rat cerebral lysosomes with autologous microsomes resulted in labilization of the lysosomal complex as assayed through determination of lysosomal acid proteinase activity in the incubate supernatant. Addition of cytosolic fraction with significant glutathione peroxidase activity largely prevented the labilization whereas an artificial scavenger of active O2 (benzoic acid) was not as effective. Frozen and thawed lysosomes served as controls. The labilization of lysosomes by microsomal O2 activation mechanism in the absence of exogenous substrate may explain neuronal degeneration in cases of induction of the oxidative activity without generation of reactive metabolites from the exogenous substrate.

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Accession: 005787243

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PMID: 435321

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