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Laboratory and clinical evaluation of a rapid slide agglutination test for phosphatidylglycerol



Laboratory and clinical evaluation of a rapid slide agglutination test for phosphatidylglycerol



American Journal of Obstetrics & Gynecology 151(8): 1061-1066



Amniostat-FLM is a recently developed rapid slide agglutination test for amniotic fluid phosphatidylglycerol. Phosphatidylglycerol quantitated by 2-dimensional TLC was compared with phosphatidylglycerol determined by Amniostat-FLM is 180 amniotic fluid samples. If any level of phosphatidylglycerol by TLC, including a trace, was considered positive, then phosphatidylglycerol by TLC and Amniostat-FLM were concordant in 171 of 180 samples (95%). Both were positive in 123 and both negative in 48 samples. The 9 amniotic fluid samples with discrepant results were as follows. Five negative Amniostat-FLM results were associated with trace phosphatidylglycerol by TLC (1-2% of total phospholipids), 1 negative Amniostat-FLM result was associated with 3% phosphatidylglycerol by TLC, and 3 positive Amniostat-FLM results were associated with negative phosphatidylglycerol by TLC results. There were 14 samples with trace phosphatidylglycerol by TLC; of these, there were 9 positive and 5 negative Amniostat-FLM results. IF > 2% phosphatidylglycerol by TLC was considered positive, concordance between the 2 tests was 93% (167 of 180). From the samples, 119 infants were delivered within 72 h of amniocentesis. There were 100 positive Amniostat-FLM results and all were associated with the absence of respiratory distress syndrome; of the 91 negative Amniostat-FLM results, 5 were associated with infants who developed respiratory distress syndrome. The present study confirms that Amniostat-FLM is a simple, rapid, and accurate test for determining the presene of phosphatidylglycerol in amniotic fluid.

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Accession: 005787312

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PMID: 3838619

DOI: 10.1016/0002-9378(85)90381-3


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