+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Laboratory and clinical investigation of recovery of Salmonella typhi from blood



Laboratory and clinical investigation of recovery of Salmonella typhi from blood



Journal of Clinical Microbiology 7(2): 122-126



An experimental study was undertaken of isolation of Salmonella typhi from artificially infected whole blood and blood clot prepared either with small numbers of freshly isolated strains or with the S. typhi Ty2 strain. Results showed that 8 ml of whole blood required a minimum of 50 ml of bile salt broth to dilute the bactericidal effect of the serum. However, the isolation rate for recovery in 210 cases of enteric fever was only 64% when 50 ml of medium was used, as compared to 92% recovery from blood clot from the same group incubated in streptokinase bile salt broth. This probably reflects a very low grade bacteremia in many of the patients. Further investigation showed that, ideally, between 150 and 200 ml of medium is required for satisfactory whole-blood culture. Both the experimental study of artificially infected clots and recovery rates from patients indicate that rapid clot dissolution with streptokinase is preferable to whole-blood culture; this experience has been amply confirmed by almost 5,000 cases over a 20-year period. A 15-ml volume of bile salt broth with 100 U of streptokinase is adequate for the clot from 8 ml of blood, and savings on media and incubator space have considerable cost benefits in developing countries.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 005787321

Download citation: RISBibTeXText

PMID: 632343


Related references

Enhanced recovery of salmonella typhi and salmonella paratyphi a in thyroxine supplemented blood cultures. Abstracts Of The Annual Meeting Of The American Society For Microbiology: 4, 1985

Evaluation of sodium poly anetholsulfonate as a blood culture additive for recovery of salmonella typhi and salmonella paratyphi a. Journal of Clinical Microbiology 18(2): 380-383, 1983

Epidemiological analysis of Salmonella typhi-murium infections on the basis of laboratory methods. I. Distribution of phage types and biotypes of Salmonella typhi-murium isolated in Hungary in the period 1960 to 1981. Acta Microbiologica Hungarica 32(1): 75-86, 1985

Pulsed-field gel electrophoresis as an epidemiologic tool in the investigation of laboratory acquired Salmonella typhi infection. Southeast Asian Journal of Tropical Medicine and Public Health 28(1): 82-84, 1997

Epidemiological analysis of Salmonella typhi-murium infections on the basis of laboratory methods. II. Resistance to antibiotics and R-plasmid carrier state in Salmonella typhi-murium isolated in Hungary in the period 1974 to 1981. Acta Microbiologica Hungarica 32(1): 87-98, 1985

Unusual clinical course of a laboratory infection caused by Salmonella typhi. Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene. 1. Abt. Medizinisch-Hygienische Bakteriologie, Virusforschung und Parasitologie. Originale 204(2): 299-301, 1967

Rapid, sensitive recovery of recombinant attenuated Salmonella enterica serovar Typhi vaccine strains from human blood. Clinical and Vaccine Immunology 20(9): 1473-1478, 2014

PCR method to identify Salmonella enterica serovars Typhi, Paratyphi A, and Paratyphi B among Salmonella Isolates from the blood of patients with clinical enteric fever. Journal of Clinical Microbiology 46(5): 1861-1866, 2008

Relative efficacy of blood, urine, rectal swab, bone-marrow, and rose-spot cultures for recovery of Salmonella typhi in typhoid fever. Lancet 1(7918): 1211-1213, 1975

Successive Salmonella give and Salmonella typhi infections, laboratory-acquired. Pathology 23(3): 233-234, 1991

Antibacterial activity of zidovudine and didanosine against blood clinical isolated of non-typhi Salmonella. Abstracts of the Interscience Conference on Antimicrobial Agents & Chemotherapy 36(0): 98, 1996

Influence of blood volume and incubation time on isolation of Salmonella typhi and Salmonella paratyphi in common blood culture media. Bangladesh Journal of Microbiology 12(1-2): 87-90, 1995

A fast and highly sensitive blood culture PCR method for clinical detection of Salmonella enterica serovar Typhi. Annals of Clinical Microbiology and Antimicrobials 9(): 14-14, 2010

Studies on salmonella strains recently isolated in hokkaido japan salmonella typhi salmonella paratyphi salmonella enteriditis salmonella schwarzengrund salmonella saint paul salmonella typhimurium salmonella monteirdeo salmonella potsdam salmonella narashino salmonella give salmonella senftenberg streptomycin anti infect tetracycline anti infect chloramphenicol anti infect. Report of the Hokkaido Institute of Public Health (16): 9-22, 1968