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Laboratory and field trials using altosid insect growth regulator against black flies diptera simuliidae of newfoundland canada

Laboratory and field trials using altosid insect growth regulator against black flies diptera simuliidae of newfoundland canada

Journal of Medical Entomology 16(6): 536-546

Altosid (methoprene), an insect growth regulator, was used in laboratory and field trials to assess its potential as a control agent for black flies (Simuliidae). Results of laboratory trials showed that Altosid caused pupal rather than larval mortality in Simulium venustum S. verecundum complex; of the several formulations tested, Altosid microencapsulated PS10 gave the best results, completely inhibiting eclosion in S. venustum S. verecundum complex at a dosage rate of 20 .mu.g/l .times. 30 min; exposure of S. vittatum larvae to Altosid PS10 at dosage rates up to 20 .mu.g/l .times. 30 min did not affect fecundity in adults; pulse (i.e., 30-min) dosing with Altosid was a more efficient means of inducing mortality than continuous dosing. Field trials were carried out using single applications of Altosid PS10 or emulsifiable concentrate at 12, 25 or 50 .mu.g/l .times. 30 min in 5 local streams. Altosid application produced a sharp drop in pupal emergence 6-7 days after dosing; the emergence rate subsequently returned gradually to predose levels. In the 1978 field program, Altosid PS10 applied at 25 or 50 .mu.g/l .times. 30 min at 14-day intervals to 3 streams inflicted 93.8-99.1% mortality in Simulium spp. and was also effective against Prosimulium mixtum.

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