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Laboratory bioassays of mixtures of bacillus thuringiensis and chitinase

Laboratory bioassays of mixtures of bacillus thuringiensis and chitinase

Canadian Entomologist 110(2): 201-204

The addition of chitinase at the rate of 1000-4000 nephelometric units/US gal to B. thuringiensis insecticide for use against spruce budworm [Choristoneura fumiferana (Clem.)] has been advocated. Bioassays of V instar spruce budworm larvae against 7 levels of Thuricide at 4 levels of chitinase corresponding to 0, 22, 220 and 2200 nephelometric units/US gal were conducted by placing larvae on balsam fir foliage (Abies balsamea L.) dipped in appropriate dilutions of Thuricide with chitinase. Mortality was assessed 6 days later. An additional replicated assay at a relatively massive dose of chitinase, 33,000 nephelometric units/US gal, was compared simultaneously with a 0 chitinase control. The data were homogeneous and linear by statistical standards. No effect of chitinase on LC50 or slope of the regression line was observed.

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Accession: 005787525

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DOI: 10.4039/ent110201-2

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