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Laboratory characteristics of the yarmouk strain of crimean congo hemorrhagic fever virus



Laboratory characteristics of the yarmouk strain of crimean congo hemorrhagic fever virus



International Journal of Zoonoses 8(2): 121-126



The Yarmouk strain of Crimean-Congo hemorrhagic fever (CCHF) virus lost 78% and 42% of its infective titers after treatment with ether and chloroform, respectively. The virus completely lost its infectivity after treatment with sodium deoxycholate. The virus was completely inactivated by heating at 56.degree., 60.degree. and 70.degree. C for 30, 10 and 5 min, respectively. Neither viral growth nor viral antigen were detected in primary lamb testis, rabbit kidney and chicken embryo fibroblast cells. The virus grew with prominent cytopathic effect in primary lamb kidney cell culture and cell lines. Experimental inoculation of sheep and goats resulted in viremia without overt disease. The virus was reisolated from the blood of inoculated animals till the 5th day postinoculation (pi). Seroconversion was observed 2-5 wk pi. Contact control animals were free of antibodies to the virus.

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Accession: 005787550

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PMID: 6806206


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